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| SHIMIZU, Takashi; AKIYAMA, Hirotada; ABE, Yasuyuki; SASADA, Hiroshi; SATO, Eimei; MIYAMOTO, Akio; UCHIDA, Takafumi; 清水, 隆; 宮本, 明夫. |
| Protein phosphorylation on certain serine or threonine residues preceding proline (Ser/Thr-Pro) is a pivotal signaling mechanism in diverse cellular processes. Pin1 is a highly conserved enzyme that isomerizes only the phosphorylated Ser/Thr-Pro bonds in certain proteins, thereby inducing conformational changes. Although much protein is phosphorylated in the ovary, the role of Pin1 in the ovary is still unknown. The purpose of this study is to investigate the effects of gonadotropins on protein and mRNA expression of Pin1 in mice ovaries. Quantitative PCR analysis showed that the expression of Pin1 mRNA significantly increased in the ovaries of equine chorionic gonadotropin (eCG)-treated mice compared with those of untreated mice (P<0.05). However,... |
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Palavras-chave: E2F; Follicle development; Gonadotropin; Ovary; Pin1. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4056 |
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| SHIMIZU, Takashi; KAWAHARA, Manabu; ABE, Yasuyuki; YOKOO, Masaki; SASADA, Hiroshi; SATO, Eimei; 清水, 隆. |
| The genetic and molecular mechanisms that control the development of capillary blood vessels during follicular development are beginning to be elucidated. Ovarian follicles contain and produce angiogenic factors that may act alone or in concert to regulate the process of thecal angiogenesis. These factors are ultimately controlled by endocrine, paracrine and autocrine regulation. A recent study indicated that vascular endothelial growth factor (VEGF) plays an important role in the process of thecal angiogenesis during follicular development. We are developing a novel technology for the induction of follicular development using the technique of in vivo gene administration. Here, we summarize the recent progress of our research. |
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Palavras-chave: Follicular development; Microvasculature; Angiogenic factor. |
| Ano: 2003 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4058 |
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| SHIMIZU, Takashi; IIJIMA, Koji; SASADA, Hiroshi; SATO, Eimei; 清水, 隆. |
| Angiogenic factors are associated with angiogenesis during follicular development in the mammalian ovary. The aim of the present study was to determine the relationships between the vascular network and mRNA expressions of angiopoietins (Ang)-1, Ang-2 and hepatocyte growth factor (HGF), and their receptors in follicles at different developmental stages during follicular development. Ovaries in gilts were collected 72 h after equine chorionic gonadotropin (eCG, 1250 IU) treatment for histological observation of the capillary network. Granulosa cells and thecal tissues in small (<4 mm), medium (4-5 mm) or large (>5 mm) individual follicles were collected for detection of mRNA expression of HGF, Ang-1 and Ang-2 in granulosa cells, and HGF receptor... |
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Palavras-chave: Follicular Development; Angiogenesis; Angiopoietins; HGF; Pig. |
| Ano: 2003 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4055 |
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